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A Bowel Flora Protocol for Dysbiosis Management
In previous
articles in the Townsend Letter a
protocol has been advanced for the management of autoimmune
diseases.1,2 A central part of this
protocol is the management and correction of intestinal dysbiosis. Phytotherapy
can play a valuable role in achieving a healthy gut flora by working
with the patient's intrinsic organisms, as outlined below.
Humans coexist in an overall symbiotic relationship with a complex array
of commensal bacterial flora that inhabits the gastrointestinal tract
(GIT). Through
processes of evolution, mammals coexist with an estimated 300 to 500 different
species of commensal bacteria that colonize the GIT in a symbiotic relationship.
In commensal relationships neither partner is harmed, in symbiotic relationships
unique metabolic traits or other benefits are provided. The presence of intestinal
bacteria plays an important role in the development of a robust intestinal
immune system and protects the host against rapid colonization by intestinal
pathogens.3,4 Lactobacilli and bifidobacteria are Gram-positive lactic acid-producing
bacteria constituting a major part of the normal intestinal microflora of humans.5,6
To ensure our GIT has sufficient defense against potential pathogenic organisms,
mucosal immune integrity and bowel flora need to be regulated as a treatment
priority.
The GIT provides distinct niches for colonization by commensal bacteria, as
indicated by qualitative and quantitative differences in the bacterial flora
throughout the GIT.3,7 The architecture in different parts of the GIT reflects
its functional role in digestion and immune response. Localized differences
are found with the presence and density of mucosal lymphoid structures such
as Peyer's patches. It is likely that the lymphoid tissue throughout
the GIT plays an important role in localized immune responses to bacteria.
Development of a strong localized gastrointestinal mucosa-associated immune
system is not only reliant on good mucosal lymphatic tissue and hence lymphatic
support, but is also functionally dependent on the presence of the bacterial
microflora.8 The gut flora can be seen as an integral part of our immune system,
and can almost be considered an organ of the human body within its own right.
It is speculated that when the bowel flora colonies become dysbiotic, autoimmune
conditions such as inflammatory bowel disease can result. (Dysbiosis is abnormal
microbial colonization of the intestine, where the changes in quality and/or
quantity are pathological.)
Bowel flora play an important role in our ability to fight infectious disease,
providing a front line in our immune defense, provide a passive mechanism to
prevent infection, and produce many vitamins. Acid-producing lactobacilli and
bifidobacteria increase the bioavailability of minerals, which require acid
for absorption — calcium, copper, iron, magnesium, manganese. Without a
healthy colony of bowel flora, we cannot expect robust health and wellbeing.
The age-old naturopathic principle, start your treatment with the gut, has
yet again been proven to hold much truth and value.
Protocol Rationale: The Weed and Feed Theory
This program has been adapted from the protocol developed by herbalist Hein
Zeylstra for the management of Crohn's disease and ulcerative colitis.
Step 1: Prepare and Weed
Prepare — Day 1: In preparation for the dysbiosis protocol, it is ideal
to have the patient fast for one day prior to commencement. For an optimal
pre-protocol 24-hour fast, advise the patient to exclude all food and beverages
other than purified water. Vegetable juices and broths are acceptable in moderation
(no more then 470 mL (16 oz) of juice during the day and ideally diluted with
some purified water).
If a patient feels that they cannot go without food for 24 hours, they can
include 1-2 servings of low glycemic vegetables, either raw or slightly steamed.
It is essential to avoid foods containing yeast, sugar and starches, including
fruit, during this 24-hour period. No alcohol or caffeine can be consumed during
the fast and ideally during the dysbiosis protocol. If cravings for carbohydrates
are interfering with patient compliance, the addition of Gymnema tablets (2-3
per day) or (even better) Gymnema liquid extract into the protocol for blood
sugar regulation will improve patient compliance.
Weed — Eradicate Dysbiotic Organisms using Garlic (Days 2-3): The main
components of garlic are the sulfur compounds, including alliin. Allicin is
produced from alliin (via the action of the enzyme allinase) when garlic is
crushed or chopped. Allicin is rather unstable and decomposes further producing
a range of compounds including diallyl sulfides, ajoenes and vinyldithiins.9
Allicin and its decomposition products are thought to be the major antimicrobial
factors in garlic.10
If fresh garlic is used in this protocol, it should be crushed first and taken
with enough water to flush the garlic through the stomach quickly so the antimicrobial
substances can act in the intestine. Enteric coated garlic tablets will ensure
that the maximum potency of garlic is delivered to the site of dysbiosis.
Garlic was used in World War I as an anti-infective agent for various infectious
intestinal diseases, including cases of cholera and dysentery. It also had
a protective antibacterial effect: soldiers whose diet included garlic suffered
less frequently from dysentery than those who did not eat garlic. In vitro
and in vivo studies indicate that garlic has both antibacterial and antifungal
activity, giving it broad spectrum antimicrobial activity in the GIT.11 Broad
spectrum antimicrobials are best for weeding as they do not create imbalance
in the microflora.
Goldenseal tablets or liquid extract equivalent to 2-3 g of goldenseal root
(in divided doses) could be added to this weeding protocol. Goldenseal, like
garlic, is a broad spectrum antimicrobial.
Other broad-spectrum antimicrobial herbs can be included in this phase. For
example, pau d'arco is an herb which possesses a broad spectrum of antimicrobial
activity, especially against protozoa and fungi, and appears to have a capacity
to kill micro-organisms, rather than merely inhibit their growth. It consists
of the inner bark of several species of Tabebuia, in particular T. avellanedae
and T. ipe. Pau d'arco contains naphthoquinones, and while much research
has focussed on lapachol, this particular compound is not the major naphthoquinone
found in the inner bark. The compound of ß-lapachone is more important
in the context of the use of the inner bark.12
Step 2: Feed (Days 4-15)
Step 2a: Provide Prebiotic to Feed the Bowel Flora with Slippery Elm powder:
The growth of endogenous beneficial bowel flora can be encouraged by administering
prebiotics. Prebiotics are food for probiotics (beneficial bowel flora),
and include herbs and foods containing mucilages, polysaccharides and fructooligosaccharides
(FOS). FOS, otherwise referred to as fructans, are complex carbohydrates
found in several common foods and a number of medicinal herbs. Foods containing
FOS include Jerusalem artichokes, globe artichoke, onions, bananas, asparagus,
leeks, garlic, wheat and barley. FOS taste sweet, however unlike sugar and
starch, they add no calories to the diet because they are not digested or
absorbed in humans. Inclusion of these in the diet can enhance GIT health
by providing an energy source for bowel flora and thereby improve nutrient
absorption and assist in reducing inflammation. FOS enhance mineral absorption
and counteract the deleterious effects of phytic acids.
The most common mucilage-containing herb historically used for GIT disorders
is slippery elm (Ulmus rubra). Slippery elm contains mucilage (a polysaccharide),
starch and minerals. The main water-soluble polysaccharide is a linear polymer
of galacturonic acid and rhamnose residues with side branches of galactose
or 3-methyl-galactose. It is demulcent, emollient and nutrient and provides
a simple physical soothing action.13,14
Mucilaginous herbs will also encourage the growth of beneficial bowel flora
and are more simple, clinically effective and inexpensive when compared to
probiotic supplementation.
Step 2b: Inhibit the Regrowth of Pathogenic Flora: Use selective gastrointestinal
antiseptics to restore normal bowel flora, such as green tea and grape seed
extract. The use of polyphenols and oligomeric procyanidins from grape seed
extract and green tea selectively inhibit the regrowth of pathogenic bowel
flora. The addition of these herbs into step 2 of the protocol improves dysbiosis
management, dramatically reduces flatulence and abdominal bloating, and provides
powerful antioxidant activity.
Green tea and grape seed contain tannins which are defined as vegetable substances
capable of tanning animal hides to produce leather. (This is used as a method
to preserve the hide and at a molecular level is effected via the crosslinking
of hide proteins by the tannins.) This definition is prescriptive and powdered
hide is still used as a phytochemical test for tannins. Like flavonoids, tannins
are polyphenolic compounds which have an affinity for proteins. However, the
higher number of phenolic groups and the larger molecular size of tannins mean
that they are capable of binding strongly to proteins at several sites and
can precipitate them from solution.
The advantage of tannins in the context of this article is that they are poorly
absorbed in the gastrointestinal tract. Hence, through their capacity to bind
proteins, they can inhibit the growth of micro-organisms, especially in the
colon. One of the most notable effects of tannins in the gut is their dramatic
effect on diarrhea. It can be proposed that the effect of tannins is to produce
a protective (if temporary) layer of coagulated protein on the mucosa along
the upper levels of the gut wall, so numbing the sensory nerve endings and
reducing provocative stimuli to additional peristaltic activity. Supporting
this central astringent activity, tannins will also inhibit the viability of
infecting micro-organisms, check fluid hypersecretion and neutralize inflammatory
proteins. Because of their affinity for free protein, they will concentrate
in damaged areas. Condensed tannins were able to bind to and inactivate the
hypersecretory activity of cholera toxin.15 Hence tannins can help to improve
gut wall integrity.
Tannins also can affect bowel flora composition. A methanol extract of green
tea was found to moderately enhance the growth of some bifidobacteria and selectively
inhibit the growth of some clostridia in vitro.6 The polyphenols containing
gallate (such as epigallocatechin gallate) had the strongest activity.16 Experimental
in vivo studies have indicated that tea catechins improve intestinal flora.
In chickens, ingestion of tea catechins resulted in a significant increase
in the number of lactobacilli and decreased Enterobacteriaceae population.
Putrefactive products also decreased. Similar results were demonstrated in
pigs.17 Grape seed oligomeric and polymeric procyanidins demonstrated a beneficial
effect on cecal fermentation in rats. Caecal pH decreased, and fermentative
activity was stimulated without an increase of deleterious enzymatic activity.18
In Japan, patented tablets containing green tea tannins are sold in pet stores
for use in dogs. Because of their effect on bowel flora they have a deodorizing
effect on dog stools which is a great boon for owners keeping indoor pets in
that country.
Green tea (a rich source of tannins) appears to be much more potent as an antimicrobial
agent than black tea.19 Bacillus subtilis, Escherichia coli, Proteus vulgaris,
Pseudomonas fluorescens, Salmonella sp. and Staphylococcus aureus were used
to test the antimicrobial activity of extracts of various tea products. Among
the six test organisms, P. fluorescens was the most sensitive to the extracts,
while B. subtilis was the least sensitive. In general, antimicrobial activity
decreased when the extent of tea fermentation increased. The antimicrobial
activities of extracts of tea products with different extents of fermentation
also varied with test organisms. Green tea, the unfermented tea, exerted the
strongest antimicrobial activity followed by the partially fermented tea products
such as Longjing, Tieh-Kuan-Ying, Paochung, and Oolong teas. On the other hand,
black tea, the completely fermented tea, showed the least antimicrobial activity.
A small clinical study in Japan demonstrated a green tea catechin preparation
was able to positively affect intestinal dysbiosis in nursing home patients
by raising levels of lactobacilli and bifidobacteria, lowering levels of Enterobacteriaceae,
Bacteroidaceae, and eubacteria, and decreasing odorous compounds. Levels of
pathogenic bacterial metabolites were also decreased.17,20 A further study
found that supplementation with tea catechins produced favorable improvements
in the participant's bowel conditions as evidenced by a reduction in
fecal moisture, pH, ammonia, sulfide and oxidation-reduction potential.21 In
both trials the dosage was 300 mg/day of tea catechins, which is equivalent
to about 6 cups of green tea.
Protocol |
Day |
Protocol |
Dietary Guidelines |
Day 1 |
Prescribed medicines and supplements are to be taken as normal
if the patient is currently on a protocol |
Fasting — no food and plenty of water; if the patient
cannot fast, recommend to eat light, fresh meals of vegetables and
salads only.
No consumption of yeast, sugar or starches is essential. This includes
fruits. Vegetable juices and broths are acceptable.
No alcohol or caffeine.
If cravings for carbohydrates are interfering with patient compliance,
add Gymnema tablets (3 per day) into the protocol for blood sugar regulation. |
Day 2 and Day 3 |
Garlic: 1-2 fresh crushed cloves of garlic twice daily or
2 high quality, enterically-coated garlic tablets. If fresh garlic
is used, it should be taken with a copious quantity of water. This
has the effect of flushing the fresh garlic quickly into the small
intestine.
Goldenseal could be taken here as well: 4 tablets containing at least 500
mg of root per day |
Fasting is ideal; if the patient cannot fast, recommend very
light, fresh meals of vegetables and salads.
No consumption of yeast, sugar or starches is essential. This includes
fruits and fruit juices. Vegetable juices and broths are acceptable.
No alcohol or caffeine. |
Days 4 to 15 |
Slippery elm powder: 1-2 heaped teaspoons of slippery elm powder
with copious (240 mL) water, to allow it to swell in the GIT.
Herbal antioxidant (green tea, grape seed extract, turmeric, rosemary): 2 tablets
at night before bed or on an empty stomach, at least 2 hours away from food |
Gradually introduce clean, fresh foods
Daily consumption of green tea |
Day 15 |
Repeat protocol for another 14 day cycle if desired |
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Case Study
A 42-year-old male presented with chronic digestive issues (probably a version
of irritable bowel syndrome) as a result of long-term antibiotic use 6 years
prior for chronic sinus infections. He had taken amoxicillin for 2 continual
years, with no improvement in his sinus health over this time.
The patient presented with chronic bloating, foul flatulence, constant nausea,
joint pain and intense itchiness of the skin, particularly of the anus. He
experienced heartburn after each meal, regardless of what he had eaten, had
marked carbohydrate cravings, despite feeling fatigue and abdominal spasms
after fruit, bread, rice and pasta consumption. He craved beer and consumed
two glasses on a daily basis. His mood fluctuated between depression and irritability.
He was about 18 kg overweight for his height, and had slowly gained the weight
over the past 10 years. He was sedentary in lifestyle, due to a lack of energy
although he wished to be more physically active.
He emptied his bowels up to four times daily, with variability in form from
looseness to constipation, accompanied by sharp pain. Urgency in both bowel
and bladder use were concerning him. His prostate was evaluated as normal in
size and function. He noted an aching pain in his kidney area and has experienced
unusual loss of patches of hair within his beard.
Comprehensive Diagnostic Stool Analysis (CDSA) results indicated positive markers
for dysbiosis and suboptimal digestion and absorption. Bacteriology revealed
no detectable growth of Lactobacillus species and a low level growth of bifidobacteria
with moderate levels of growth of streptococcus, Hafnia alvei and E. coli.
He was positive for Helicobacter pylori. Inflammation markers were high, but
no ova or parasites were detected. Urinary organic acid testing revealed poor
fatty acid and carbohydrate metabolism, poor citric acid cycle (resulting in
key symptom of depression and fatigue) with high inflammation and intestinal
dysbiosis markers of bacteria, yeast and fungi. The patient was also exhibiting
classic features of adrenal fatigue (which was not confirmed at this stage
by testing.)
The following treatment was advised.
Week 1 |
Diet: Fast and consume only steamed or lightly stir
fried nonstarchy vegetables, with a focus on the Brassica group;
no alcohol and caffeine
Garlic tablets: 3 tablets per day on the weekends
Slippery elm powder: 1 rounded tablespoon mixed briskly into 200 mL purified
water morning and night on weekday only
Herbal antioxidant (green tea, grape seed extract, turmeric, rosemary): 2 tablets
at night before bed during the weekdays only |
Weeks 2—6 |
Garlic tablets: 3 tablets per day on the weekends
Slippery elm powder: 1 rounded tablespoon mixed briskly into 200 mL purified
water morning and night on weekday only
Herbal antioxidant (green tea, grape seed extract, turmeric, rosemary): 2 tablets
at night before bed during the weekdays only
Diet: Anticandida dietary guidelines (no sugar, starch, refined carbohydrates,
caffeine or alcohol) |
After the initial 7 days on the protocol, the bowel flora program was
repeated, with the addition of an herbal formula to further support the
mucosal immunity in the GI tract. The anticandida diet was to be implemented
for 12 weeks.
Herbal Formula |
Goldenseal (Hydrastis canadensis) |
1:3 |
30 mL |
Uva ursi (Arctostaphylos uva-ursi) |
1:2 |
40 mL |
Gymnema (Gymnema sylvestre) |
1:1 |
40 mL |
Calendula (Calendula officinalis) |
1:2 |
30 mL |
Licorice high grade (Glycyrrhiza glabra) |
1:1 |
20 mL |
Echinacea angustifolia and E. purpurea root blend |
1:2 |
20 mL |
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200 mL (total) |
Dose: 8 mL twice daily 15 minutes before meals. |
Treatment Rationale
With the positive diagnosis of advanced intestinal dysbiosis the first step
was the implementation of a bowel flora protocol. As the sinus infections
were unresolved with antibiotic therapy it was possible they were due to
chronic fungal infection, compounded by food sensitivity due to low GI mucosal
immunity.
- Goldenseal was included for its trophorestorative action on the mucous
membranes of both the GI and sinus, and for its antimicrobial activity.
- Uva
ursi was included for its high tannin content, as an antibacterial
to inhibit the growth of Hafnia alvei in the GIT.
- Gymnema was selected
to manage pancreatic function and assist in the reduction of sugar
cravings. Gymnema can dramatically improve patient dietary
compliance, which is central to long-term management of dysbiosis.
- Calendula
was incorporated as an anti-inflammatory and vulnerary for the GIT.
- Licorice
was added also to reduce the inflammatory response, to soothe the irritated
mucous membranes and to support adrenal function.
- Echinacea root blend
to improve mucosal immunity and as a general immune modulator, given
the history of recurrent infections.
In addition to the benefit in the bowel flora protocol the turmeric
in the herbal antioxidant tablet provides liver support for detoxification
of
the endotoxin liberation from bacterial and fungal die off. This helps
in reducing
the associated muscle ache, headaches and nausea.
Second Consultation (6 weeks later)
The patient had achieved remarkable improvement. He had lost 11 kg in weight
as a result of dysbiosis management, frequency of bowel motions improved
(twice daily) and occurred without any pain. Stools were of normal color
and reduced odor; flatulence had improved by approximately 80% and there
was no odor to the gas. The rectal itching had resolved, as had the itchiness
of the skin. Reflux had improved dramatically after 3-4 weeks in the program.
The sinuses were less congested, such that he could breathe clearly at night.
As a result of better quality sleep and adrenal support, he was waking feeling
refreshed and had more sustained energy throughout the day. The aching in
the kidney area was resolved, as was most of the joint ache.
Despite the difficulty in the first week of the program with the dietary changes
and fasting, he now has a more controlled appetite, yet able to eat more and
feel less full after eating. His craving for carbohydrates had dissipated.
The protocol remained unchanged for a further 6 weeks before CDSA and urinary
organic acid analysis was performed.
Conclusion of Care
Test results came back illustrating a significant improvement in bowel flora
and resultant digestive health. There was strong growth of both Lactobacillus
species and bifidobacteria with negligible levels of growth of streptococcus,
Hafnia alvei and E. coli. He was now testing negative for Helicobacter pylori.
Inflammation markers were now within normal ranges.
He is maintained on the following:
- Herbal antioxidant: 2 tablets each night
before bed to inhibit the regrowth of pathogenic flora, anti-inflammatory,
liver support and antioxidant for general
preventative medicine.
- Echinacea angustifolia and E. purpurea root: 2 tablets
each morning for general immune support.
- Tablets containing Withania
somnifera and Panax ginseng: 2 tablets a day for adrenal support
and stress management.
Ms. Angela Hywood ND, Dip Herb, MNHAA
Angela Hywood graduated in Australia in 1995 as a Naturopath, Herbalist
and Homeopath. After several years of general practice, Angela completed
postgraduate
studies in the fields of Fertility Management; Women & Children's
Health Care and specialized clinical practice in these fields. She studied
for three years at the School of Pharmacy, Curtin University of Technology,
Australia and has a family history spanning back two generations in the field
of pharmacy. For four years, Angela was a faculty member of both the Botanical
and Naturopathic Medicine departments of several Australian naturopathic
schools. She is currently a faculty member of postgraduate education at several
departments in the US. Over the past three years, Angela has been a featured
speaker at a number of medical, naturopathic, chiropractic, and herbal medicine
conferences in the US and Australia. She currently works in the US as a consultant
for Standard Process and MediHerb.
References
1. Bone K. Autoimmune disease: A phytotherapeutic perspective. Townsend
Letter for Doctors & Patients 1999 Aug/Sep; #193/194: 94-98
2. Bone K. Phytotherapy for autoimmune disease: A focus on multiple
sclerosis. Townsend Letter for Doctors & Patients 2004; #250 [in
press]
3. Hooper LV, Gordon JI. Science 2001; 292: 1115-1118
4. Guarner F, Malagelada JR. Lancet 2003; 361: 512-519
5. Orrhage K, Nord CE. Drugs Exp Clin Res 2000; 26(3): 95-111
6. Ahn YJ, Sakanaka S, Kim MJ et al. Microb
Ecol Health Dis 1990; 3:
335-338
7. Jiang HQ, Bos NA, Cebra JJ. Infect Immun 2001; 69: 3611-3617
8. Yamanaka T, Helgeland L, Farstad IN et al. J
Immunol 2003; 170: 816-822
9. British Herbal Medicine Association. British
Herbal Compendium, Volume 1. BHMA, Bournemouth, 1992, pp 105-106.
10. Abdullah TH, Kandil O, Elkadi A et al. J
Natl Med Assoc 1988; 80(4):
439-445
11. Koch PH, Lawson LD (eds). Garlic: The Science
and Therapeutic Application of Allium sativum L. and Related Species, 2nd Edition. Williams & Wilkins,
Baltimore, 1996, pp 164-172.
12. Mills S, Bone K. Principles and Practice
of Phytotherapy: Modern Herbal Medicine. Churchill Livingstone, Edinburgh, 2000, pp 499-506.
13. British Herbal Medicine Association. British
Herbal Compendium, Volume 1. BHMA, Bournemouth, 1992, p 204.
14. Mills SY. The A-Z of Modern Herbalism. Thorsons, London, 1989, p
194.
15. Hšr M, Rimpler H, Heinrich M. Planta
Med 1995; 61: 208-212
16. Ahn YJ, Kawamura T, Kim M et al. Agric Biol
Chem 1991; 55(5): 1425-1426
17. Hara Y. J Cell Biochem Suppl 1997; 27: 52-58
18. Tebib K, Besancon P, Rouanet JM. Nutr Res 1996; 16(1): 105-110
19. Chou CC, Lin LL, Chung KT. Int J Food Microbiol 1999; 48: 125-130
20. Goto K, Kanaya S, Nishikawa T et al. Ann
Long-Term Care 1998; 6:
1-7
21. Goto K, Kanaya S, Ishigami T et al. J Nutr
Sci Vitaminol 1999; 45(1):
135-141
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