I am writing this
to reveal the instructions for making Coley's
Toxins, a simple procedure. First, however, I want to offer a bit of
history on Coley's Toxins.
In 1890, Dr. William Coley was a 28-year-old surgeon in New York City.
Much of Coley's history, detailed here, comes from Stephen Hall's
book, A Commotion in the Blood (NY: Henry Holt
and Company; 1997).
In 1890, Bessie Dashiell, a young woman in her late teens, came to Dr. Coley
with a painful injury to one hand.
The injury eventually proved to be round cell sarcoma. Coley amputated her
hand, but the cancer spread throughout her body, and she died a horrible death.
Coley was shaken by the woman's death and decided there had to be a
better way to treat cancer. He spent many hours going through the records of
New York Hospital until he found a clue. In 1885, a poor German immigrant named
Stein had had surgery for round cell sarcoma four times, and his case was considered
hopeless. Then he was infected with erysipelas. He nearly died from the infection,
but when he recovered, the cancer was gone. Coley searched for Stein throughout
the lower East Side ghetto until at last, six years later, he found Stein — in
the best of health.
At that point, Coley decided to infect late-stage cancer patients with erysipelas.
His first test case was a patient named Zola. Zola's case nearly duplicated
Stein's experience. Coley had much trouble getting an infection started
with Zola and, when he did, it was so severe that he feared Zola would die
of the infection. Yet, as in the case of Stein, Zola went into a remission
that proved to be a cure.
Coley began to treat twelve late-stage cancer patients by infecting them with
erysipelas. Two patients died from the infection. One patient suffered from
an infection with a fever of 105°F. In that case, the patient's
tumors disappeared, but a recurrence caused death, Coley was unable to infect
the other nine patients.
Coley turned to a erysipelas vaccine, killed by heating to 66°C for two
hours. This vaccine caused a mild fever reaction, but not one strong enough
to work as an anticancer treatment. Coley added the newly discovered Bacillus
prodigiosus — now called Serratia marcescens — to the vaccine. That
was killed by heating to 66°C. This was and is Coley's Toxins
and was injected into a tumor or done as intradermal injections. First, the
patient experienced cold, followed by a fever of 101° to 104° for
a few hours.
The first patient to be treated with Coley's Toxins was a boy named
John Ficken. He had a large tumor of malignant sarcoma in the abdomen that
had been like a bowling ball. His condition was considered hopeless. On January
24, 1893, the new Coley's Toxins was injected into the tumor. The reactions
to the injections were severe chills and fever. The injections were done every
other day for five months. After this time, the boy's tumor had decreased
by 80%, and Coley stopped treatment. The boy was gaining back lost weight.
He was sent home at this time and was traced in 1907, still alive and well.
Coley had had a success with his first cancer patient treated with Coley's
Coley started to treat cancer patients with Coley's Toxins with a degree
of success. Soon, other doctors were beginning to get Coley's Toxins
from Coley. Despite numerous successes, on December 15, 1894, an editorial
appeared in the American Medical Association's publication, the Journal
of the American Medical Association, stating that Coley's Toxins was
useless in cancer treatment. By the spring of 1896, however, Coley had
treated 160 cancer patients with his toxins. Nearly half of these 160 patients
had shown a degree of benefit; for a few of them, the results had been nothing
short of remarkable. In 1897, Coley took the post of head of the Sarcoma Ward
at Memorial Hospital in New York City. Meanwhile, the Mayo Brothers had come
out as strong advocates for Coley's Toxins in treating cancer.
One of Dr. Coley's colleagues was Dr. James Ewing, a pathologist who
came to Memorial Hospital about the same time as Coley. In time he was known
to the news media as Mr. Cancer, and he developed for Coley and his toxins
the most bitter animosity. He thought that the newly discovered radium and
the X-ray machine was the Utopian cure for cancer.
Ewing attracted the interest of James Douglas, CEO of the Phelps-Dodge Corp.
Douglas had a daughter with breast cancer. Ewing, Douglas, and his daughter
went to England where she could be treated with radium. The treatment failed,
and she died of breast cancer, this after the first breast cancer patient cure
by Coley's Toxins as has been reported. In a letter to Ewing, however,
Douglas was not swayed from Ewing's methods by the death of his daughter.
He formed a firm for the mining of radium and made a gift of several hundred
thousand dollars worth of radium to Memorial Hospital. In return for the gift,
Ewing was made Medical Director of Memorial Hospital. Ewing became Coley's
boss and his worst enemy. As a result, for many years, Coley could not treat
sarcoma patients with Coley's Toxins; he was only permitted to treat
cancer patients using radium — with poor results.
Meanwhile, not far from Memorial Hospital, one of the most dramatic cures of
cancer by Coley's Toxins took place. The patient was an officer in the
Merchant Marine. The time was 1926. The patient had reticulum cell sarcoma.
His leg had been amputated at the hip. Three months later, a metastasis appeared
above the umbilicus. One month later, a fist-sized tumor had developed on the
amputated stump. And one month after that, the amputated stump was increasing
in size, and another orange size tumor had appeared on the stump.
Coley's Toxins were injected into the stump. There were signs of regression,
but the reactions were severe, and the patient asked for a respite. During
the respite, the cancer spread at an alarming rate. The stump increased in
size to 30 inches in circumference, and the end of the stump had broken down
to a great ulcer from which a foul-smelling discharge emanated. Several more
tumors appeared, these in the scalp, the vertebrae, and the cranial bones.
Injections were begun again in the stump. A large dose of Coley's Toxins
was injected every day. The patient suffered severe reactions, but after 28
days, the ulcer in the stump healed, and the stump had returned to its normal
size. All his other tumors had either vanished or were now very small in size.
His doctors wanted to continue with the treatment, but the patient said that
he had had enough. Sixty days later, he showed no sign of cancer. The patient
lived free of cancer until 1959, when he died of a heart attack.
In 1935, Coley gave a lecture at the University of Glasgow in which he told
of treating several hundred cancer patient with sarcoma. Of them, about one
third had remissions that left them free of cancer at five years. For a long
time, Dr. Ernest Codman of the Harvard Medical School had sided with Ewing
in saying that Coley's Toxins were useless in the treatment of cancer,
but in 1935, one year before the death of Coley, he changed his position and
said that the use of Coley's Toxins had produced from time to time miracles
in cancer treatment. His observation did no good. Coley died the next year,
and, for a time, Coley's Toxins died with him.
Coley's daughter Helen Coley Nauts founded the Cancer Research Institute
in 1953, and it has grown large and prestigious. In 1956, she had Coley's
Toxins made at the Memorial Sloan Kettering Cancer Center, Coley's old
hospital, and she offered it to doctors for cancer treatment. Helen Coley Nauts
found good records of just less than 1,000 cancer patients who had been treated
with Coley's Toxins. Of them, about half had remissions that left them
free from cancer at five years.
The American Cancer Society, founded in 1913, has been a bitter enemy of Coley's
Toxins. In June 1962, an amendment to the Pure Food and Drug act was passed,
giving great power to the FDA. At this time, the American Cancer Society had
a list of unproven treatments of cancer, and Coley's Toxins was on that
list. The American Cancer Society held that Coley' s Toxins had never
helped a cancer patient The act was called the Kefauver-Harris Amendment and
contained a grandfather clause that made legal any drug or vaccine that had
a record of success before 1962. Aspirin was at once made legal, but with the
American Cancer Society designation of Coley's Toxins as ineffective
with a cancer patients, Coley's Toxins was declared illegal.
Ironically, that designation appeared even as Coley's Toxins continued
to find success with patients. In February 1961, for instance, a retired contractor
was dying at Baptist Hospital in Oklahoma City. The patient had had surgery
for colon cancer. There followed a massive reoccurrence. The liver was enlarged
and there were metastases in the peritoneum and lungs. His doctor was lapping
and removing two quarts of bloody fluid from the abdomen each day and one quart
of fluid from the lungs every second day. The fluid from both the lungs and
abdomen had cancer cells. Death was expected in a week or so.
Otradermal injections of Coley's Toxins was started in the buttock.
The patient experienced aches and pains and shaking chills, along with a fever
of 102°. The injections were done for eight days only. By then, the miracle
had taken place. Twenty-four hours after the first injection, no more fluid
remained in the lungs. After three days, there was no more fluid to be removed
from the abdomen. On day eight of the injections, the patient was feeling fine,
and the injections were stopped. A week later, the patient was gaining lost
weight and he was sent home. He was alive and well in 1970, nine years later.
Although other cancer patients were being treated with Coley's Toxins
and showing benefit, their treatment suddenly stopped after June of 1962 when
the FDA outlawed the use of Coley's Toxins.
The make-up of Coley's Toxin's is remarkably simple. From 1894
until 1906, Coley's Toxins was made by Dr. B.H. Buxton of Cornell University.
He soaked one pound of ground beef over night in 1000 cc of water. Then he
boiled the beef for one hour before filtering it through cotton cloth. At that
point, he added ten grams of peptone and five grams of sodium chloride, then
tested the mixture with litmus to get the solution slightly alkaline. He boiled
the solution for one hour, filtered it through filter paper, then boiled it
again for a half-hour, once a day, three days in a row. The solution was then
seeded with a live streptococcus solution and let stand for ten days. The solution
was covered, but the growth needed air. The solution became cloudy. Then a
few cc of the live Bacillus prodigiosus was added to the solution and allowed
to grow for ten days more. At that point, the solution was heated for two hours
at 58°C, and a bit of thymol was added. The vaccine was stored at 2°C
From 1906 until 1920, Dr. Martha Tracy — who had worked with Buxton — changed
the way the vaccine was made. She grew the two bacteria separately. Then she
did a nitrogen determination on the B. prodigiosus and, depending on what she
found, she added a certain amount of Bacillus prodigiosus to the streptococcus
growth. I do not like this way of making the vaccine. If one makes a mistake
in doing the nitrogen determination and gets too much of the B. prodigiosus,
the vaccine will turn very toxic. Making the vaccine as per Buxton, one will
never get a toxic vaccine.
In 1990, I had a call from Don Carrow, MD in Tampa, Florida. Dr. Carrow wanted
to know how to make Coley's Toxins. I sent him the above information,
Rather than using beef broth, he used as a broth Difco AOAC, a product of Difco
Laboratories in Detroit. In 1000 cc of water, he added 15 grams of Difco AOAC,
10 grams of Bacto peptone — another Difco product — five grams of sodium
chloride and 100 grams of glucose, He got the ph from 7.1 to 7.2. Dr. Carrow
then added a few cc of live streptococcus solution and let it stand at 36°C
for ten days. He got the 1,000 cc to 25°C and seeded it with live Serratia
marcescens and let it grow for another ten days. At that point, the vaccine
was heated to 65°C for two hours to get a killed vaccine, and Carrow
added 0.03 cc per cc of benzyl alcohol. The vaccine was stored at 2°C
to 4°C. The 1,000 cc was then filtered through a seven micron filter
with care taken not to remove the dead bacteria.
He had a cancer patient ready to treat with his Coley's Toxins. A 50-year-old
nurse with non-Hodgkin's lymphoma, this patient had a tumor under one
arm that was the size of a football. He injected his Coley's Toxins
into the center of that big tumor with a three inch long needle. Injections
were done each day. These produced first shaking chills, then a fever of 104°F.
I do not know how many injections were given, but the tumor was reduced to
a flabby bag that was removed by surgery. The bag contained no cancer cells.
Dr. Carrow reported in 2002, shortly before his own death, that the patient
Other doctors also have had recent success with Coley's Toxins. One
doctor in Ohio shared with me his procedure for making Coley's Toxins.
He reported this vaccine has effected two complete remissions of cancer. This
doctor got the live Streptococcus pyogenes, ATTC #196 15, and the live Serratia
marcescens, ATTC #13880, from MicroBiologics (www.microbiologics.com) in the
form of Kwik-Stics. For a broth, he used the Todd-Hewitt broth. It was obtained
as a powder and is used as a three-percent solution. He used 500 cc of water.
To it was added 15 grams of the Todd-Hewitt powder and 50 grams of glucose.
The flask was plugged with a plastic foam stopper and autoclaved for 15 minutes
at 121°C. The broth was allowed to cool and was stored at 4°C for
three weeks. The broth was then seeded with the live streptococcus and allowed
to grow at 36°C for seven days. The culture was then gotten to 25°C
and was seeded with the live Serratia marcescens and allowed to grow for seven
more days, The culture then was killed by heating to 65°C for two hours.
Then 7.5 cc of benzyl alcohol was added. The 500 cc of the killed vaccine was
then filtered with a 15 micron filter. It was stored at 4°C, ready for
Another doctor, whom I'll call Doctor Y, has added a new dimension to
the treatment of cancer with Coley's Toxins. He sets up an IV of Coley's
Toxins in his office for his patients. He then shows the patients how to do
the injections at home as self-medication and sends them home with a 20 cc
bottle of Coley's Toxins. He instructs the patient to have rectal suppositories
of Tylenol on hand, since rectal suppositories will terminate a reaction to
Coley's Toxins quickly. The patient is to do an injection at about 8:00
AM. For an hour, he cautions, the patient will feel cold and may shake. Then
the fever will come on and the pulse will increase to about 125. The patient
is told to check temperature and pulse every hour. If the fever exceeds 104°F,
or if the pulse exceeds 135, the patient should terminate the reaction with
Tylenol. This will not happen often. The fever should end by 6:00 PM.
We should be able to do better than Coley did. He did not have Tylenol. He
knew that an overdose of Coley's Toxins could cause death. so he had
to use lower doses than he would have preferred. Having Tylenol on hand permits
the use of higher doses. In Coley's time, too, a hard-and-fast rule
declared that nothing must be put in a vein. Now we understand that injections
of Coley's Toxins in the vein give greater reactions. The injections
can be done every day or every second day. The injections are done for 30 days,
halted for a week, then resumed.
All these instructions are given to the patient. Sometimes the treatment needs
be done for two or three months. By doing the injections at home, the patient
can get two months of treatment that could not be had otherwise. and the cost
is kept down. Dr. Y has had two complete remissions of cancer and two failures.
Helen Coley Nauts has told me that doctors at the Cancer Research Institute
feel that the main anticancer effect of Coley's Toxins lies in the anticancer
effects of fever and interferon and the tumor necrosis factor, all of which
are increased during the reaction. Professor Leo Zacharski, MD, Dartmouth Medical
School says that Coley's Toxins contains some streptokinase. In a recent
communication, he said he knew of a woman with endometrial cancer with metastases.
She had a heart attack and was treated with an infusion of streptokinase. The
streptokinase terminated the heart attack, and the patient's tumors
showed a marked regression which has persisted for several months. He was hoping
to see a trial on treating cancer with an infusion of streptokinase, but meanwhile
he suggested that streptokinase in Coley's Toxins was part of its anticancer
effect. I also made contact with Dr. S. Moncath, Medical Director of Wellcome
Research Laboratories. He said that, in the fever reaction to Coley's
Toxins or to an infection of erysipelas, the arginine-to nitric-oxide pathway
is activated for the production of nitric oxide, which provides a strong anticancer
I hope that, by offering this history of Coley's Toxins, along with
examples of doctors who have had recent success treating cancer with Coley's
Toxins, I will encourage other doctors to make Coley's Toxin's
and use it to treat cancer patients.
Wayne Martin, BS, ChE
25 Orchard Court
Fairhope, Alabama 36532 USA